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                                                                                                   shRNA慢病毒包裝
服務介紹

慢病毒(Lentivirus) 載體是以HIV-1 (人類免疫缺陷Ⅰ型病毒)為基礎發展起來的基因治療載體。區別一般的逆轉錄病毒載體,它對分裂細胞和非分裂細胞均具有感染能力。

慢病毒載體可以將外源基因或外源的shRNA有效地整合到宿主染色體上,從而達到持久性表達目的序列的效果。在感染能力方面可有效地感染神經元細 胞、肝細胞、心肌細胞、腫瘤細胞、內皮細胞、干細胞等多種類型的細胞,從而達到良好的的基因治療效果。對于一些較難轉染的細胞, 如原代細胞、干細胞、不分化的細胞等,使用慢病毒載體,能大大提高目的基因或目的shRNA的轉導效率,且目的基因或目的shRNA整合到宿主細胞基因組 的兒率大大增加,能夠比較方便快捷地實現目的基因或目的shRNA的長期、穩定表達。

所以,在體外實驗及體內實驗的研究中,慢病毒己經成為表達外源基因或外源shRNA的常用載體形式之一, 并且正在獲得越來越廣泛的應用。

服務內容

- 广东快乐十分直播开奖
實驗方案 服務描述 滴度 價格 服務周期
1 客戶提供靶點序列,我們構建到指定載體,包裝慢病毒 1x108 TU 2500元/靶點病毒 2-3周
2 客戶根據目的基因從我們shRNA慢病毒庫中選擇shRNA現貨質粒,我們包裝成慢病毒 1x108 TU 2500元/靶點病毒 2 - 3 周
3 客戶提供基因名稱,我們負責設計,提供客戶3個靶點慢病毒,保證干擾效率在RNA水平70%以上 1x108 TU 8800元/套病毒 3 - 4 周
4 客戶根據目的基因從我們的shRNA慢病毒文庫中選擇質粒,我們提供質??寺?,客戶自行驗證效率后挑選有效靶點包裝慢病毒 1x108 TU 1200元/套質粒 1周

載體信息(可在我公司載體庫中查看詳情)

載體名稱 抗性 報告基因
plko.1-TRC Puro /
plko.1 puro Puro /
plko.3G Puro /
pLVX-sh1 Puro /
pLKO-puro-TurboGFP Puro TurboGFP
pLVX-sh2 / EGFP
pLVX-U6-RFP-Puro Puro RFP
pLVX-U6-GFP-Puro / GFP
pLVX-U6-EGFP Puro EGFP
pLVX-U6-tdTomato Puro tdTomato
pLVX-U6-YFP Puro YFP
pLVX-U6-Neo Neo /
pLVX-U6-Neo-EGFP Neo EGFP
pLVX-U6-Puro-Tet-on Puro  

備注:本公司慢病毒系統包裝質粒為PSPAX2 及PMD2G

訂購流程

1.質量保證 2.發貨時間 3.關于售后
Transheep 有一套成熟的質檢程序,產品發貨前均經過質量檢測,確保您收到的產品質量無誤! Transheep擁有大量的基因現貨產品,本公司的現貨產品從訂貨日期起一周以內即可到貨,大大縮短了您的等待時間!另外Transheep的一站式克隆平臺也能讓您體驗到前所未有的快速定制服務! Transheep所售出的產品全部登記在冊,如果您拿到我們的產品覺得和實質不相符或存在問題,您只需要告訴我們相關產品信息我們會迅速做出反應,為您提供解決方案或退款!
4.訂購流程 5.聯系我們 6.相關服務:
聯系我們→客戶或銷售人員與您溝通→簽訂合同→支付預付款→執行項目→確認收貨→支付尾款→售后服務 Transheep提供免費的技術咨詢,任何關于產品或者技術問題,都可以聯系我們們。 免費熱線:400-0179890 服務郵箱:[email protected] 基因合成
亞克隆構建
3,UTR驗證實驗
啟動子合成及驗證
慢病毒包裝
腺病毒包裝
穩轉株構建
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