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                                                                                         shRNA腺病毒包裝

服務介紹

腺病毒是一種線性雙鏈DNA病毒,由于其具有宿主范圍廣、可感染分裂和非分裂細胞、高滴度制備、外源基因裝載容量大等優點,重組腺病毒已越來越廣泛地被應用于基礎研究和臨床試驗中,并成為世界上最常用的基因治療載體之一。
權陽生物重組腺病毒系統是將攜帶外源基因的腺病毒穿梭質粒與攜帶腺病毒基因組的包裝質粒共轉染包裝細胞,借助Cre/loxP系統的作用重組產生腺病毒。該系統既克服了傳統共轉染系統重組效率低的缺點,也避免了細菌重組中的種種不確定性,實現了快速高效的腺病毒包裝。
權陽生物提供的重組腺病毒為復制缺陷型病毒,即病毒感染目的細胞后不會再感染其他細胞,也不會利用宿主細胞產生新的病毒顆粒,安全性能良好。
腺病毒介導的shRNA可高效感染到各種原代細胞、動物模型及組織中,表達效率極高。

服務流程

1.    腺病毒穿梭載體的構建:
將設計好的shRNA構建到RNAi的腺病毒載體;
2.     腺病毒包裝過程:
將腺病毒穿梭質粒及其輔助包裝質粒共轉染至包裝細胞中,通過Cre/loxP系統的作用實現重組,產生重組腺病毒顆粒。

實驗方案 服務描述 滴度 價格 服務周期
1 客戶提供靶點序列,我們構建到指定RNAi腺病毒載體,包裝腺病毒,非純化。 1x10E10 pfu 6500元/個病毒 6-8周
2 客戶提供基因名稱,我們負責設計,提供客戶3個靶點非純化腺病毒,保證干擾效率在RNA水平70%以上。 1x10E10 pfu 12500元/套病毒 6-8 周
3 客戶提供靶點序列,我們構建到指定RNAi腺病毒載體,包裝腺病毒,純化至1x10E11 pfu,可用于體內實驗。 1x10E11 pfu 11500元/個病毒 8 - 10 周
4 客戶提供基因名稱,我們負責設計,提供客戶3個靶點非純化腺病毒,客戶自行驗證效率,保證干擾效率在RNA水平70%以上,驗證好的目的病毒我們純化至1x10E11 pfu,可用于體內實驗。 1x10E11 pfu 17500元/套病毒 8 - 12 周


服務流程

1.質量保證 2.發貨時間 3.關于售后
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4.訂購流程 5.聯系我們 6.相關服務:
聯系我們→客戶或銷售人員與您溝通→簽訂合同→支付預付款→執行項目→確認收貨→支付尾款→售后服務 Transheep提供免費的技術咨詢,任何關于產品或者技術問題,都可以聯系我們們。 免費熱線:400-0179890 服務郵箱:[email protected] 基因合成
亞克隆構建
3,UTR驗證實驗
啟動子合成及驗證
慢病毒包裝
腺病毒包裝
穩轉株構建

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