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                                           細菌16S rDNA擴增引物

核糖體RNA(rRNA)是生物細胞中主要的核糖核酸之一。rRNA分子的一級結構既具有高度保守性區域,又具有高度變化特異性區域。保守區域反映了生物物種間的親緣關系,可用于構建生命統一進化樹,而高度變化特異區域則能表明物種間的差異,可用于區別屬或者種。

16S rRNA位于原核細胞核糖體小亞基上,是細菌分類學研究中最常用的"分子鐘",其序列包含10個保守區域和9個高度變化特異性,其中保守區在細菌間差異不 大,高度變化性特異性區域具有屬或種的特異性,序列則隨菌間的親緣關系不同而有一定的差異。因此,16S rRNA基因是能揭示生物物種的特征核酸序列,被認為是最適于細菌系統發育和分類鑒定的指標。

16S rDNA基因測序,通常是選擇某個或某幾個變異區域,選擇通用引物進行擴增成PCR產物,然后進行測序分析和菌種鑒定。通過檢測16S rDNA的序列變異和豐度,可以了解環境樣品中群落多樣性信息?;?6S rDNA的分析在微生物分類鑒定,微生態研究等方面起到重要作用。

18S rDNA或ITS(Internal Transcribed Spacer)被廣泛應用在真菌分類鑒定中。18S rDNA在系統發育研究中較適用于種級以上階元的分類。ITS屬于中度保守的區域,利用它可研究種及種以下的分類階元。

B-V1-9A-F(10 nmol)

5'-GAGTTTGATCATGGCTCAG-3'

B-V1-9C-F(10 nmol)

5'-GAGTTTGATCCTGGCTCAG-3'

B-V3-541R(10 nmol)

5'-WTTACCGCGGCTGCTGG-3'

W=A or T

B-V6-967F(10 nmol)

5'-CNACGCGAAGAACCTTANC-3'

N=A or G or C or T

B-V6-1046R(10 nmol)

5'-CGACAGCCATGCANCACCT-3'

B-V9-U1406R(10 nmol)

5'-CGGAACATGTGTGGCGGGCAG-3'

B-V9-N1406R(10 nmol)

5'-AACGTGAGTGGCGGGCA-3'

B-V9-UA1406R(10 nmol)

5'-AACRTGWGTGGCGGGCA-3'

R=A or G

W=A or T

B-V9-1541R(10 nmol)

5'-AAGGAGGTGATCCANCCRCA-3'

N=A or G or C or T

R=A or G

引物服務和訂購:

根據您的需要請選擇相應的合成服務

Email訂購:請填寫引物合成訂購表(下載附件),聯系地址:[email protected]

發貨形式: 凍干粉或液體交貨

發貨COA 文件包括產品:
                                              1).引物序列;
                                              2). OD值;
                                              3).Tm 等詳細信息數據。

 

【參考文獻】 

Nam Y-D, Jung M-J, Roh SW, Kim M-S, Bae J-W. (2011) Comparative Analysis of Korean Human Gut Microbiota by Barcoded Pyrosequencing. PLoS ONE 6(7): e22109.

Baker GC, Smith JJ, Cowan DA (2003) Review and re-analysis of domain specific 16S primers. J Microbiol Methods 55: 541–555.

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