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慢病毒包裝試劑盒
發布日期:2015-10-03 19:39:05 瀏覽次數 :
導讀
Lenti-TSB™ 慢病毒包裝試劑盒Transheep的的Lenti-TSB™ 表達包裝系統包括優化的慢病毒包裝質粒
  Lenti-TSB™ 慢病毒包裝試劑盒    
           
  Transheep的的Lenti-TSB™ 表達包裝系統包括優化的慢病毒包裝質粒,有多種Tag及啟動子可供選擇copGFP陽性對照質粒,Namipo-T新型轉染試劑等。該系統與的Transheep的ORF、shRNA克隆配套使用保證更高滴度和更高表達水平,同時為您降低可觀的實驗成本。    
           
    內容 價格    
  慢病毒空載 豐富的載體可供選擇,包括不同的熒光、抗性、啟動子 800元起    
  模版合成 如果您沒有目的基因的模版,我們可以幫您合成任意序列并克隆到您選定的載體 低至1元/bp    
  載體構建        構建任意您感興趣的基因到您選擇的載體,包括LncRNA等,最大容量為6kb 800元起    
  表達檢測 構建好的載體少量抽提,轉染工具細胞進行目的基因表達檢測,保證您收到的載體可高效表達 500元起    
  包裝體系  經過優化的高純度包裝質粒體系,按推薦比例與穿梭質?;旌霞純?/td> 500元起    
   質粒抽提  去內毒素高純度質粒是慢病毒包裝的必備條件,Transheep可提供質粒制備及專用的抽提試劑盒 500元起    
  轉染試劑 Namipo-T™ ,納米材料293t專用新型轉染試劑,效率可達90%以上,幾乎無毒,更利于出毒 800元起     
  包裝及滴度檢測 提供包裝好的純化慢病毒,保證滴度達到要求 2000元起    
           
實驗流程     
  構建目的基因的慢病毒載體,制備慢病毒穿梭質粒及其輔助包裝原件載體質粒,分別進行高純度無內毒素抽提,使用Namipo-T共轉染 293T 細胞,培養 48和 72h 后,分別收集富含慢病毒顆粒的細胞上清液,病毒上清液通過超離心濃縮病毒。    
實驗材料     
           
(1)慢病毒載體、包裝細胞和菌株    
  Transheep的的Lenti-TSB™ 表達包裝系統,可根據您的需求現在最適合的表達載體,多種啟動子可選,CMV、PGK 啟動子在常規腫瘤細胞有極高的表達能力,而 MSVC、EF1 啟動子則可滿足干細胞中過表達基因的需求。Puro、Neo、 抗性,copGFP、tRFP、Luc 等多種抗性基因與熒光標記的選擇搭配。
可實現多種基因的共轉化。出更高的出毒效率。
   
       
貨號 類別 載體名稱 熒光標記 抗性 蛋白標簽
TSE01 慢病毒OE pTSBOE-CMV-MSC-3flag-EF1-copGFP 綠色 Flag
TSE02 慢病毒OE pTSBOE-CMV-MSC-3flag-EF1-copGFP-F2A-Puro 綠色 Puro Flag
TSE03 慢病毒OE pTSBOE-CMV-MSC-3flag-EF1-tRFP-F2A-Puro 紅色 Puro Flag
TSE04 慢病毒OE pTSBOE-CMV-MSC-3flag-EF1-copGFP-F2A-Neo 綠色 Neo Flag
TSE05 慢病毒OE pTSBOE-CMV-MSC-3flag-EF1-tRFP-F2A-Neo 紅色 Neo Flag
TSE06 慢病毒OE pTSBOE-CMV-MSC-EF1-Puro Puro
TSE07 慢病毒OE pTSBOE-CMV-MSC-IRES-Neo Neo
TSE08 慢病毒SH pTSBSH-U6-MSC-CMV-ZsGreem-PGK-Puro 綠色 Puro  
TSE09 慢病毒SH pTSBSH-U6-MSC-CMV-ZsGreem-PGK-Neo 綠色 Puro  
TSE10 慢病毒SH pTSBSH-U6-MSC-CMV-tRFP-PGK-Puro 紅色 Neo  
TSE11 慢病毒SH pTSBSH-U6-MSC-CMV-tRFP-Neo 紅色 Neo  
TSE12 慢病毒SH pTSBSH-U6-MSC-CMV-Neo Neo  
TSE13 慢病毒SH pTSBSH-U6-MSC-CMV-Puro Puro  
TSE14 慢病毒SH pTSBSH-H1-MSC-FE1-Puro-copGFP 綠色 Puro  
TGL004 慢病毒 pTsLVGL-MCS-Minp-Luc2-EF1-Puro LUC2 Puro 轉錄因子、啟動子驗證
TGL005 慢病毒 pTsLVGL-CMV-Luc2-EF1-Puro LUC2 Puro UTR驗證,啟動子驗證
     
(2) 包裝體系    
  Transheep的 packing mix是我們經過優化過的包裝體系,可以按照穿梭質粒與包裝體系(1+基因大小kb)/1的比例混合即可,Lenti-TSB™也可以使用通用的包裝細胞進行包裝。    
(3)菌株大腸桿菌菌株    
  推薦使用top10作為質粒擴增及構建的菌株。如果基因復雜可考慮使用stbl3菌株,保真度要高一些,正常情況下使用top10引起載體突變的概率不大。    
(4)包裝細胞      
  TSB-293Tmax™ 細胞系轉染效率高,能夠實現病毒蛋白高水平的表達,表現出更高的出毒效率。    
(5)試劑耗材        
  慢病毒包裝所用的質粒必須去內毒素處理,培養耗材及血清也是影響慢病毒出毒的一大因素,市場上的試劑假冒產品較多,可咨詢客戶購買適合包裝所用的相關產品。    
           
實驗流程    
  請參考TSB慢病毒包裝試劑盒使用說明    
       
參考文獻    
  The antihelminthic drug niclosamide effectively inhibits the malignant phenotypes of uveal melanoma in vitro and in vivo;Theranostics. 2017; 7(6): 1447–1462.
Published online 2017 Apr 3. doi:  10.7150/thno.17451
   
  USP19 suppresses cellular type I interferon signaling by targeting TRAF3 for deubiquitination;Published Online:10 Apr 2017https://doi.org/10.2217/fmb-2017-0006    
  Upregulation of the Non-Coding RNA OTUB1-isoform 2 Contributes to Gastric Cancer Cell Proliferation and Invasion and Predicts Poor Gastric Cancer Prognosis;Int J Biol Sci. 2016; 12(5): 545–557.
Published online 2016 Mar 3. doi:  10.7150/ijbs.13540
   
  Pristimerin effectively inhibits the malignant phenotypes of uveal melanoma cells by targeting NF?κB pathway;Published online on: July 25, 2017   https://doi.org/10.3892/ijo.2017.4079    
  Multifunctional nanoparticles for co-delivery of paclitaxel and carboplatin against ovarian cancer by inactivating the JMJD3-HER2 axis;The article was received on 21 Jun 2017, accepted on 31 Jul 2017 and first published on 03 Aug 2017    
  Long non-coding RNA LOC389641 promotes progression of pancreatic ductal adenocarcinoma and increases cell invasion by regulating E-cadherin in a TNFRSF10A-related manner;https://doi.org/10.1016/j.canlet.2015.12.010    
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